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product specification of |
Catalase-Peroxidase of Synechocystis PCC6803 |
General Product Information |
| Product name: | Catalase-Peroxidase of Synechocystis PCC6803 | |||
| Description: | Bifunctional haem enzyme with catalase and peroxidase activtiy |
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| Form: | Brown solid, lyophilized from 10 mM phosphate buffer, pH 7.0. |
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| Solubility: | Reconstitution of 200 µg with 240 µl distilled water gives approximately 10 µM (per haem) Catalase-Peroxidase calculated with a molar extinction coefficient at 406 nm of 100 000 M-1cm-1 |
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| Storage: | Stored
below 0°C this lyophilized product is stable for years. |
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| Source: | Overexpressed in Escherichia coli as soluble protein containing a hexa-histidine tag and purified by metal chelate affinity chromatography and gel filtration. FOR RESEARCH ONLY! |
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| EC Number: | 1.11.1.7 |
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| Molecular weight: | ~ 170 000 Da (Homodimer) ( see MALDI-TOF Spectrum ) |
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| Purity: | > 99% |
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| Reinheitszahl: | A406 / A280 ³ 0.55 ( see UV / VIS Spectrum ) |
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| Specific activity: | 970 |
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1000 |
units per mg (catalase activity, hydrogen peroxide) |
for further information about the assays have a look at detailed unit definition |
Detailed Unit
Definition |
| Assay
1 (Catalase activity) |
Assay
2 (Peroxidase activity) |
Assay 1 (Catalase activity): |
One unit of catalase activity catalyzes the formation of 1 µmol of O2 per minute at 30°C. |
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| Total reaction volume: | 5 ml
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Final assay concentrations: |
67 mM
phosphate buffer, pH 7.0
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References: |
Rorth, M. and Jensen, P.K. (1967) Biochim. Biophys. Acta 139, 171-173 |
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| Assay
1
(Catalase activity) |
Assay
2
(Peroxidase activity) |
Assay 2 (Peroxidase activity): |
One unit of peroxidase activity catalyzes the oxidation of 1 µmol of o-dianisidine per minute at 25°C.
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Total reaction volume: |
1 ml
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Final assay concentrations: |
67 mM
phosphate buffer, pH 7.0 |
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References: |
Mutsuda M., Ishikawa T., Takeda T. and Shigeoka S. (1996) Biochem J. 316: 251-257 | |
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