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Catalase-Peroxidase of Synechocystis PCC6803

 

General Product Information


Product name: Catalase-Peroxidase of Synechocystis PCC6803
Description:

Bifunctional haem enzyme with catalase and peroxidase activtiy

Form:

Brown solid, lyophilized from 10 mM phosphate buffer, pH 7.0.

Solubility:

Reconstitution of 200 µg with 240 µl distilled water gives approximately 10 µM (per haem) Catalase-Peroxidase calculated with a molar extinction coefficient at 406 nm of 100 000 M-1cm-1

Storage:

Stored below 0°C this lyophilized product is stable for years.

Source:

Overexpressed in Escherichia coli as soluble protein containing a hexa-histidine tag and purified by metal chelate affinity chromatography and gel filtration.

FOR RESEARCH ONLY!
NOT FOR HUMAN OR DRUG USE!

EC Number:

1.11.1.7

Molecular weight:

~ 170 000 Da  (Homodimer)  ( see MALDI-TOF Spectrum )

Purity:

> 99%

Reinheitszahl:

A406 / A280 ³ 0.55  ( see UV / VIS Spectrum )

Specific activity:

970
1.5



1000
2.5

units per mg (catalase activity,  hydrogen peroxide)
units per mg (peroxidase activity,  o-dianisidine)

for further information about the assays have a look at detailed unit definition


Detailed Unit Definition



 

Assay 1
(Catalase activity)
Assay 2
(Peroxidase activity)

 

Assay 1 (Catalase activity):


One unit of catalase activity catalyzes the formation of 1 µmol of O2 per minute at 30°C.

Total reaction volume: 5 ml

 

Final assay concentrations:

67 mM phosphate buffer, pH 7.0
5 mM hydrogen peroxide

 

References:

Rorth, M. and Jensen, P.K. (1967) Biochim. Biophys. Acta 139, 171-173

 

 

 

 

 

 

 

Assay 1
(Catalase activity)
Assay 2
(Peroxidase activity)

 

Assay 2 (Peroxidase activity):


One unit of peroxidase activity catalyzes the oxidation of 1 µmol of o-dianisidine per minute at 25°C.

 

Total reaction volume:

1 ml

 

Final assay concentrations:

67 mM phosphate buffer, pH 7.0
1 mM hydrogen peroxide
1 mM o-dianisidine
 

References:

Mutsuda M., Ishikawa T., Takeda T. and Shigeoka S. (1996) Biochem J. 316: 251-257

 

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